{"id":7974,"date":"2018-04-07T12:57:57","date_gmt":"2018-04-07T12:57:57","guid":{"rendered":"https:\/\/sc.qu.edu.iq\/\/?p=7974"},"modified":"2024-02-24T17:19:22","modified_gmt":"2024-02-24T14:19:22","slug":"%d8%af%d8%b1%d8%a7%d8%b3%d8%a9-%d9%88%d8%a8%d8%a7%d8%a6%d9%8a%d8%a9-%d9%84%d8%a8%d9%83%d8%aa%d8%b1%d9%8a%d8%a7-listeria-monocytogenes-%d8%a7%d9%84%d9%85%d8%b3%d8%a8%d8%a8%d8%a9-%d9%84%d9%84%d8%a7","status":"publish","type":"post","link":"https:\/\/sc.qu.edu.iq\/?p=7974","title":{"rendered":"\u062f\u0631\u0627\u0633\u0629 \u0648\u0628\u0627\u0626\u064a\u0629 \u0644\u0628\u0643\u062a\u0631\u064a\u0627 Listeria monocytogenes \u0627\u0644\u0645\u0633\u0628\u0628\u0629 \u0644\u0644\u0627\u062c\u0647\u0627\u0636 \u062f\u0631\u0627\u0633\u0629 \u0644\u0637\u0627\u0644\u0628\u0629 \u0641\u064a \u0643\u0644\u064a\u0629 \u0627\u0644\u0639\u0644\u0648\u0645"},"content":{"rendered":"<p style=\"text-align: right\" align=\"CENTER\"><span style=\"font-size: 12pt\"><span lang=\"ar-IQ\"><b>\u00a0\u0639\u0644\u0649 \u0642\u0627\u0639\u0629 \u0623\u0628\u0646 \u0627\u0644\u0647\u064a\u062b\u0645 \u0641\u064a \u0643\u0644\u064a\u0629 \u0627\u0644\u0639\u0644\u0648\u0645 \u0628\u062c\u0627\u0645\u0639\u0629 \u0627\u0644\u0642\u0627\u062f\u0633\u064a\u0629 \u062c\u0631\u062a \u0645\u0646\u0627\u0642\u0634\u0629 \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u0627\u0644\u0645\u064f\u0639\u064e\u062f\u0629 \u0645\u0646 \u0642\u0628\u0644 \u0627\u0644\u0637\u0627\u0644\u0628\u0629 <span style=\"color: #0000ff\">\u0639\u0637\u0627\u0621 \u062e\u0644\u064a\u0644 \u062d\u0633\u064a\u0646<\/span> \u062a\u062d\u062a \u0639\u0646\u0648\u0627\u0646\u00a0<\/b><span style=\"font-size: 14pt\"><b><span dir=\"RTL\" lang=\"AR-IQ\" style=\"line-height: 115%;font-family: 'Times New Roman', serif\">\u062f\u0631\u0627\u0633\u0629 \u062a\u0634\u062e\u064a\u0635\u064a\u0629 \u0648\u062c\u0632\u064a\u0626\u064a\u0629 \u0644\u0628\u0643\u062a\u0631\u064a\u0627 <\/span><i><span style=\"line-height: 115%;font-family: 'Times New Roman', serif\">Listeria monocytogenes<\/span><\/i><span dir=\"RTL\" lang=\"AR-IQ\" style=\"line-height: 115%;font-family: 'Times New Roman', serif\"> \u0627\u0644\u0645\u0639\u0632\u0648\u0644\u0629 \u0645\u0646 \u0645\u0635\u0627\u062f\u0631 \u0645\u062e\u062a\u0644\u0641\u0629 \u0641\u064a \u0645\u062d\u0627\u0641\u0638\u062a\u064a \u0628\u0627\u0628\u0644 \u0648\u0627\u0644\u062f\u064a\u0648\u0627\u0646\u064a\u0629.<\/span><\/b><\/span><b>\u00a0<span style=\"color: #0000ff\">\u0628\u0623\u0634\u0631\u0627\u0641 \u0623.<\/span><\/b><\/span><span lang=\"ar-IQ\"><b><span style=\"color: #0000ff\">\u062f \u0645\u064a\u062b\u0645 \u063a\u0627\u0644\u064a \u064a\u0648\u0633\u0641<\/span> \u2026 \u0648\u062a\u0646\u0627\u0648\u0644\u062a \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u0627\u0644\u062a\u064a<\/b><\/span><strong>\u0627\u062c\u0631\u064a\u062a<\/strong>\u00a0<strong>\u062e\u0644\u0627\u0644 \u0627\u0644\u0641\u062a\u0631\u0629 \u0645\u0646 \u062a\u0634\u0631\u064a\u0646 \u0627\u0644\u062b\u0627\u0646\u064a 2016 \u0627\u0644\u0649 \u0627\u064a\u0627\u0631 2017, \u062c\u0645\u0639 \u0627\u0644\u0639\u064a\u0646\u0627\u062a \u0627\u0644\u0633\u0631\u064a\u0631\u064a\u0629 \u0645\u0646 \u0627\u0644\u0646\u0633\u0627\u0621 \u0627\u0644\u0644\u0648\u0627\u062a\u064a \u064a\u0639\u0627\u0646\u064a\u0646 \u0645\u0646 \u062d\u0627\u0644\u0627\u062a \u0627\u0644\u0627\u062c\u0647\u0627\u0636 \u0636\u0645\u0646 24 \u0627\u0633\u0628\u0648\u0639 \u062e\u0644\u0627\u0644 \u0641\u062a\u0631\u0629 \u0627\u0644\u062d\u0645\u0644. \u0627\u0630 \u062c\u0645\u0639\u062a \u0627\u0644\u0639\u064a\u0646\u0627\u062a \u062a\u062d\u062a \u0627\u0634\u0631\u0627\u0641 \u0637\u0628\u064a \u0645\u062a\u062e\u0635\u0635 \u0648\u0643\u0627\u0646 \u0639\u062f\u062f \u0627\u0644\u0639\u064a\u0646\u0627\u062a \u0627\u0644\u0645\u0623\u062e\u0648\u0630\u0629 92 \u0639\u064a\u0646\u0629 \u0633\u0631\u064a\u0631\u064a\u0629 \u0645\u0646 \u0645\u0633\u062a\u0634\u0641\u0649 \u0628\u0627\u0628\u0644 \u0644\u0644\u0646\u0633\u0627\u0626\u064a\u0629 \u0648\u0627\u0644\u0627\u0637\u0641\u0627\u0644 \u0627\u0644\u062a\u0639\u0644\u064a\u0645\u064a \u0648 83 \u0639\u064a\u0646\u0629 \u0633\u0631\u064a\u0631\u064a\u0629 \u0645\u0646 \u0645\u0633\u062a\u0634\u0641\u0649 \u0627\u0644\u062f\u064a\u0648\u0627\u0646\u064a\u0629 \u0644\u0644\u0646\u0633\u0627\u0626\u064a\u0629 \u0648\u0627\u0644\u0627\u0637\u0641\u0627\u0644 \u0627\u0644\u062a\u0639\u0644\u064a\u0645\u064a \u062d\u064a\u062b \u062a\u0636\u0645\u0646\u062a \u0627\u0644\u0639\u0632\u0644\u0627\u062a \u0639\u064a\u0646\u0627\u062a \u0627\u0644\u0645\u0634\u064a\u0645\u0629 \u0648\u0627\u0644\u0645\u0633\u062d\u0627\u062a \u0627\u0644\u0645\u0647\u0628\u0644\u064a\u0629. \u0641\u064a \u062d\u064a\u0646 \u062c\u0645\u0639\u062a \u0639\u064a\u0646\u0627\u062a \u0627\u0644\u0627\u063a\u0630\u064a\u0629 \u0628\u0635\u0648\u0631\u0629 \u0639\u0634\u0648\u0627\u0626\u064a\u0629 \u0645\u0646 \u0627\u0633\u0648\u0627\u0642 \u0641\u064a \u0645\u0646\u0627\u0637\u0642 \u0645\u062e\u062a\u0644\u0641\u0629 \u0641\u064a \u0645\u062d\u0627\u0641\u0638\u062a\u064a \u0628\u0627\u0628\u0644 \u0648\u0645\u062d\u0627\u0641\u0638\u0629 \u0627\u0644\u062f\u064a\u0648\u0627\u0646\u064a\u0629 \u0648\u0627\u0644\u062a\u064a \u0634\u0645\u0644\u062a (69) \u0639\u064a\u0646\u0629 \u062c\u0628\u0646 , (58) \u0639\u064a\u0646\u0629 \u0644\u062d\u0648\u0645 \u062d\u0645\u0631\u0627\u0621 \u0645\u062c\u0645\u062f\u0629 \u0648 (51) \u0639\u064a\u0646\u0629 \u0644\u062d\u0648\u0645 \u062f\u062c\u0627\u062c \u0645\u062c\u0645\u062f\u0629.<\/strong><\/span><\/p>\n<p><span style=\"font-size: 12pt\"><strong>\u062a\u0645 \u0639\u0632\u0644 \u0628\u0643\u062a\u0631\u064a\u0627 <em>Listeria monocytogenes<\/em> \u0628\u0623\u0633\u062a\u062e\u062f\u0627\u0645 \u0648\u0633\u0637 \u0632\u0631\u0639\u064a \u0627\u062e\u062a\u064a\u0627\u0631\u064a \u062e\u0627\u0635 \u0628\u0628\u0643\u062a\u0631\u064a\u0627 <em>L. monocytogenes<\/em> \u0645\u0639 \u0627\u0636\u0627\u0641\u0629 \u0627\u0644\u0645\u0639\u0632\u0632 \u0627\u0644\u062d\u064a\u0648\u064a \u0627\u0644\u0630\u064a \u064a\u0633\u0627\u0639\u062f \u0639\u0644\u0649 \u0646\u0645\u0648\u0647\u0627 \u0648\u062a\u062b\u0628\u064a\u0637 \u0646\u0645\u0648 \u0627\u0644\u0627\u0646\u0648\u0627\u0639 \u0627\u0644\u0627\u062e\u0631\u0649 \u0645\u0646 \u0627\u0644\u0628\u0643\u062a\u0631\u064a\u0627, \u0648\u0634\u062e\u0635\u062a \u0627\u0644\u0639\u0632\u0644\u0627\u062a \u0628\u0623\u0633\u062a\u062e\u062f\u0627\u0645 \u0627\u0644\u0641\u062d\u0635 \u0627\u0644\u0645\u062c\u0647\u0631\u064a \u0648\u0627\u0644\u0627\u062e\u062a\u0628\u0627\u0631\u0627\u062a \u0627\u0644\u0643\u064a\u0645\u0648\u062d\u064a\u0648\u064a\u0629. \u0627\u0638\u0647\u0631\u062a \u0627\u0644\u0646\u062a\u0627\u0626\u062c \u0627\u0646 \u0646\u0633\u0628\u0629 \u0627\u0644\u0639\u0632\u0644 \u0644\u0628\u0643\u062a\u0631\u064a\u0627 <em>L. monocytogenes<\/em> \u0643\u0627\u0646\u062a 13(3.68%)\u00a0 \u0639\u0632\u0644\u0629 \u0645\u0646 \u0645\u062c\u0645\u0648\u0639 353 \u0639\u064a\u0646\u0629 \u0648\u0634\u0645\u0644\u062a :6(3.42%) \u00a0\u00a0\u0639\u0632\u0644\u0627\u062a \u0645\u0646 \u0645\u062c\u0645\u0648\u0639 175 \u0639\u064a\u0646\u0629 \u00a0\u0633\u0631\u064a\u0631\u064a\u0629 \u0648 7(3.93%) \u0639\u0632\u0644\u0627\u062a \u0645\u0646 \u0645\u062c\u0645\u0648\u0639 178 \u00a0\u0645\u0646 \u0639\u064a\u0646\u0627\u062a \u0627\u0644\u0627\u063a\u0630\u064a\u0629, \u062d\u064a\u062b \u0643\u0627\u0646\u062a \u0646\u0633\u0628\u0629 \u0627\u0644\u0639\u0632\u0644 \u0641\u064a \u0645\u062d\u0627\u0641\u0638\u0629 \u0628\u0627\u0628\u0644 3(3.26%) \u0639\u0632\u0644\u0629 \u0645\u0646 \u0645\u062c\u0645\u0648\u0639 92 \u0639\u064a\u0646\u0629 \u0648 \u0641\u064a \u0645\u062d\u0627\u0641\u0638\u0629 \u0627\u0644\u062f\u064a\u0648\u0627\u0646\u064a\u0629 3(3.61%) \u0645\u0646 \u0645\u062c\u0645\u0648\u0639 83 \u0639\u064a\u0646\u0629. \u0643\u0645\u0627 \u0643\u0627\u0646\u062a \u0646\u0633\u0628\u0629 \u0639\u0632\u0644 \u0627\u0644\u0628\u0643\u062a\u0631\u064a\u0627 \u0645\u0646 \u0627\u0644\u0627\u063a\u0630\u064a\u0629 3(4.35%) \u0645\u0646 \u0645\u062c\u0645\u0648\u0639 69 \u0639\u064a\u0646\u0629 \u062c\u0628\u0646, \u0648 2(3.92%) \u0645\u0646 \u0645\u062c\u0645\u0648\u0639 51 \u0645\u0646 \u0639\u064a\u0646\u0627\u062a \u0644\u062d\u0648\u0645 \u0627\u0644\u062f\u062c\u0627\u062c \u0648 2(3.44%) \u0645\u0646 \u0639\u064a\u0646\u0627\u062a \u0627\u0644\u0644\u062d\u0648\u0645 \u0627\u0644\u062d\u0645\u0631\u0627\u0621.<\/strong><\/span><\/p>\n<p><span style=\"font-size: 12pt\"><strong>\u0643\u0645\u0627 \u0628\u064a\u0646\u062a \u0646\u062a\u0627\u0626\u062c \u0647\u0630\u0647 \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u0627\u0646 \u0627\u0643\u062b\u0631 \u0641\u0626\u0629 \u0639\u0645\u0631\u064a\u0629 \u0645\u0639\u0631\u0636\u0629 \u0644\u0644\u0627\u0635\u0627\u0628\u0629 \u0628\u0639\u062f\u0648\u0649 \u0628\u0643\u062a\u0631\u064a\u0627 <em>L. monocytogenes<\/em> \u0647\u064a (26-30) \u0633\u0646\u0629 \u062d\u064a\u062b \u0643\u0627\u0646\u062a \u062a\u0645\u062b\u0644 \u0646\u0633\u0628\u0629 ( 3(5.17% \u0648 \u0643\u0627\u0646\u062a \u0627\u0644\u0646\u0633\u0627\u0621 \u0627\u0644\u0623\u0643\u062b\u0631 \u0627\u0635\u0627\u0628\u0629 \u0644\u0639\u062f\u0648\u0649 \u0627\u0644\u0628\u0643\u062a\u0631\u064a\u0627 \u062e\u0644\u0627\u0644 \u0627\u0644\u0623\u0634\u0647\u0631 \u0627\u0644\u062b\u0644\u0627\u062b\u0629 \u0627\u0644\u0623\u0648\u0644\u0649 (\u0623\u0642\u0644 \u0645\u0646 12 \u0623\u0633\u0628\u0648\u0639 \u0645\u0646 \u0639\u0645\u0631 \u0627\u0644\u062d\u0645\u0644) \u0643\u0627\u0646\u062a \u0628\u0646\u0633\u0628\u0629 5(5.15%) \u0648\u0643\u0627\u0646 \u0623\u0643\u062b\u0631 \u0627\u0644\u0645\u0631\u0636\u0649 \u0625\u0635\u0627\u0628\u0629 \u0645\u0646 \u0627\u0644\u0645\u0646\u0627\u0637\u0642 \u0627\u0644\u0631\u064a\u0641\u064a\u0629 4(4.39%) .<\/strong><\/span><\/p>\n<p><span style=\"font-size: 12pt\"><strong>\u0627\u0633\u062a\u062e\u062f\u0645\u062a \u062a\u0642\u0646\u064a\u0629 (PCR) Polymerase chain reaction \u0644\u062a\u0623\u0643\u064a\u062f \u062a\u0634\u062e\u064a\u0635 \u0639\u0632\u0644\u0627\u062a \u0628\u0643\u062a\u0631\u064a\u0627 <em>L. monocytogenes<\/em> \u0645\u0646 \u062e\u0644\u0627\u0644 \u0627\u0644\u0643\u0634\u0641 \u0639\u0646 \u062c\u064a\u0646\u0627\u062a \u0627\u0644\u0636\u0631\u0627\u0648\u0629 (<em>hlyA, prfA, inlA, actA, plcA, iapA<\/em>), \u0627\u0630 \u0643\u0627\u0646\u062a \u0646\u0633\u0628\u0629 \u0638\u0647\u0648\u0631 \u0647\u0630\u0647 \u0627\u0644\u062c\u064a\u0646\u0627\u062a 100% \u0644\u062c\u0645\u064a\u0639 \u0639\u0632\u0644\u0627\u062a \u0628\u0643\u062a\u0631\u064a\u0627 <em>L. monocytogenes<\/em>.<\/strong><\/span><\/p>\n<p style=\"text-align: right\" align=\"CENTER\"><span style=\"font-size: 12pt\"><strong>\u00a0\u0643\u0645\u0627 \u062a\u0645 \u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u062a\u0642\u0646\u064a\u0629 \u062a\u0633\u0644\u0633\u0644 \u0627\u0644\u062d\u0627\u0645\u0636 \u0627\u0644\u0646\u0648\u0648\u064a DNA sequencer \u0648\u062a\u062d\u0644\u064a\u0644 \u0627\u0644\u0634\u062c\u0631\u0629 \u0627\u0644\u0648\u0631\u0627\u062b\u064a\u0629 Phylogenetic tree analysis \u0639\u0644\u0649 \u0627\u0633\u0627\u0633 \u062a\u0633\u0644\u0633\u0644 \u062c\u064a\u0646 (<em>hlyA<\/em>) \u0627\u0644\u0630\u064a \u064a\u0639\u062a\u0628\u0631 \u0627\u0644\u062c\u064a\u0646 \u0627\u0644\u062a\u0634\u062e\u064a\u0635\u064a \u0644\u0628\u0643\u062a\u0631\u064a\u0627 <em>\u00a0L. monocytogenes<\/em> \u0639\u0646 \u0628\u0627\u0642\u064a \u0627\u0646\u0648\u0627\u0639 <em>Listeria spp.<\/em> \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0628\u0631\u0646\u0627\u0645\u062c (MEGA 6) \u0648\u0643\u0627\u0646 \u062a\u062d\u0644\u064a\u0644 \u0627\u0644\u0634\u062c\u0631\u0629 \u0627\u0644\u0648\u0631\u0627\u062b\u064a\u0629 \u0627\u0644\u0645\u0633\u062a\u062e\u062f\u0645 \u0645\u0646 \u0646\u0648\u0639 (UPGMA tree ), \u0644\u062e\u0645\u0633 \u0639\u0632\u0644\u0627\u062a \u0645\u0646 \u062e\u0645\u0633 \u0645\u0635\u0627\u062f\u0631 \u0639\u0632\u0644 \u0645\u062e\u062a\u0644\u0641\u0629 \u0642\u064a\u062f \u0627\u0644\u062f\u0631\u0627\u0633\u0629. \u0627\u0638\u0647\u0631\u062a \u0646\u062a\u0627\u0626\u062c \u0627\u0644\u062a\u062d\u0644\u064a\u0644 \u0627\u0644\u0648\u0631\u0627\u062b\u064a \u0648\u062c\u0648\u062f \u062a\u0642\u0627\u0631\u0628 \u062c\u064a\u0646\u064a \u0628\u064a\u0646 \u0627\u0644\u0639\u0632\u0644\u0627\u062a \u0627\u0644\u0645\u062d\u0644\u064a\u0629 \u0648\u0627\u0644\u0639\u0632\u0644\u0627\u062a \u0627\u0644\u0645\u0633\u062c\u0644\u0629 \u0639\u0627\u0644\u0645\u064a\u0627 \u0641\u064a \u0628\u0646\u0643 \u0627\u0644\u062c\u064a\u0646\u0627\u062a \u0643\u0645\u0627 \u0627\u0639\u0637\u062a \u0646\u062a\u0627\u0626\u062c \u0627\u0644\u062a\u062d\u0644\u064a\u0644 \u0627\u0644\u062c\u064a\u0646\u064a \u0648\u062c\u0648\u062f \u0646\u0633\u0628\u0629 \u062a\u0642\u0627\u0631\u0628 \u062c\u064a\u0646\u064a \u0628\u064a\u0646 \u0627\u0644\u0639\u0632\u0644\u0627\u062a \u0627\u0644\u063a\u0630\u0627\u0626\u064a\u0629 \u0627\u0643\u062b\u0631 \u0645\u0642\u0627\u0631\u0646\u0629 \u0645\u0639 \u0627\u0644\u0639\u0632\u0644\u0627\u062a \u0627\u0644\u0633\u0631\u064a\u0631\u064a\u0629 \u0643\u0645\u0627 \u0627\u0638\u0647\u0631\u062a \u0646\u062a\u0627\u0626\u062c \u0627\u0644\u062a\u062d\u0644\u064a\u0644 \u0627\u0644\u062c\u064a\u0646\u064a \u0644\u0646\u0633\u0628\u0629 \u0627\u062d\u062a\u0645\u0627\u0644\u064a\u0629 \u062d\u062f\u0648\u062b \u0627\u0644\u0637\u0641\u0631\u0627\u062a Transition substitution mutation \u00a0\u0627\u0643\u062b\u0631 \u0645\u0646 \u062d\u062f\u0648\u062b \u0637\u0641\u0631\u0627\u062a Transversion substitution mutation \u0636\u0645\u0646 \u062a\u062a\u0627\u0628\u0639 \u062a\u0633\u0644\u0633\u0644 \u0627\u0644\u062c\u064a\u0646\u0627\u062a \u0628\u064a\u0646 \u0627\u0644\u0639\u0632\u0644\u0627\u062a \u0627\u0644\u0645\u062d\u0644\u064a\u0629 \u0648\u0627\u0644\u0639\u0632\u0644\u0627\u062a \u0627\u0644\u0645\u0633\u062c\u0644\u0629 \u0639\u0627\u0644\u0645\u064a\u0627.<\/strong><\/span><\/p>\n<p align=\"CENTER\"><span style=\"font-size: 12pt\">______________________________________<\/span><\/p>\n<p style=\"text-align: left\" align=\"CENTER\"><span style=\"font-size: 12pt\"><strong>The study was conducted during the period from January 2016 to\u00a0<\/strong><strong>May 2017. Clinical samples were collected from women suffering from\u00a0<\/strong><strong>miscarriage before 24 weeks of gestation,the samples were collected<\/strong><strong>under specialized medical supervision.<\/strong><strong>The number of samples taken was 92 clinical samples from<\/strong><strong>Babylon Teaching Hospital of Gynecology and Pediatrics and 83 clinical\u00a0<\/strong><strong>samples from Maternal and Children Hospital in Al- Qadisiyah provinice.\u00a0<\/strong><strong>Food samples were randomly collected from supermarkets in different\u00a0<\/strong><strong>areas of Babil and Qadisiyah governorates, which included 69 samples of<\/strong><\/span><br \/>\n<span style=\"font-size: 12pt\"> <strong>cheese, 58 frozen red meat, 51 frozen chicken meat samples.\u00a0<\/strong><strong>Listeria monoytogenes were isolated using a selective media L.<\/strong><strong>monocytogenes, with the addition of a supplement that helps to grow L.<\/strong><\/span><br \/>\n<span style=\"font-size: 12pt\"> <strong>monocytogenes and inhibit the growth of other types of bacteria. The\u00a0<\/strong><strong>isolates were identified using microscope and biochemical tests. The\u00a0<\/strong><strong>results showed that the isolation rate of L. monoytogenes was 13 (3.68%)<\/strong><strong>of the total number of 353 isolates and 6 (3.42%) isolates out of 175\u00a0<\/strong><strong>clinical samples and 7 (3.93%) isolates out of 178 food samples, In Babil\u00a0<\/strong><strong>province 3 (3.26%) isolation from the total of 92 samples and in AlQadisiyah\u00a0<\/strong><strong>province 3 (3.61%) of the total of 83 samples. 3 (4.35%) of<\/strong><strong>total 69 samples of cheese, 2 (3.92%) of 51 chicken meat samples and 2\u00a0<\/strong><strong>(3.44%) of 58 red meat samples were isolated.<\/strong><strong>The results of this study demonstrated that the most age group was\u00a0<\/strong><strong>exposed to L. monoytogenes infection (26-30), which represented 3 of\u00a0<\/strong><strong>total 6 (5.17%). The women were most infected with bacteria during the\u00a0<\/strong><strong>first three months (less than 12 weeks of gestational age) 5 of total 6\u00a0<\/strong><strong>(5.15%) were the most infected patients of rural areas 4 of total 6(4.39%).\u00a0<\/strong><strong>PCR technique was used to confirm the diagnosis of L. monoytogenes\u00a0<\/strong><strong>isolates by detecting the virulence genes (hlyA, prfA, inlA, actA, plcA,\u00a0<\/strong><strong>iapA), as the percentage of the appearance of these genes was 100% for<\/strong><\/span><br \/>\n<span style=\"font-size: 12pt\"> <strong>all isolates of L. monoytogenes.\u00a0<\/strong><strong>DNA sequencer technique and phyloggenetic tree analysis were based on\u00a0<\/strong><strong>hlyA gene sequence clearly detection L. monocytogenes, could be the\u00a0<\/strong><strong>reliable option to indicate presence of L. monocytogenes. In the present\u00a0<\/strong><strong>study, we identified 5 isolates of L. monocytogenes from clinical and food\u00a0<\/strong><strong>samples based on hlyA gene sequence. Using MEGA 6 software, and\u00a0<\/strong><strong>UPGMA tree analysis (Unweighted Pair Group Method with Arithmetic\u00a0<\/strong><strong>Mean).\u00a0<\/strong><strong>Our results demonstrate the relationship between the local isolates with\u00a0<\/strong><strong>the other Gene Bank isolates, and also showed a genetic affinity in the\u00a0<\/strong><strong>isolates of L. monocytogenes isolated from food samples compared to the<\/strong><\/span><br \/>\n<span style=\"font-size: 12pt\"> <strong>clinical isolates. Also showed a higher probability of transition\u00a0<\/strong><strong>substitution mutation than transversion substitution mutation within the\u00a0<\/strong><strong>sequence of gene between local isolates and globally recognized isolates.<\/strong><\/span><\/p>\n<p align=\"CENTER\"><span style=\"font-size: 12pt\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-7975\" src=\"https:\/\/sc.qu.edu.iq\/\/wp-content\/uploads\/2018\/04\/image-0-02-04-3e1015e47bca414abd932ee49fae576a84f8849c0d70c3721bc65f7918d3def3-V-300x225.jpg\" alt=\"\" width=\"300\" height=\"225\" srcset=\"https:\/\/sc.qu.edu.iq\/wp-content\/uploads\/sites\/41\/2018\/04\/image-0-02-04-3e1015e47bca414abd932ee49fae576a84f8849c0d70c3721bc65f7918d3def3-V-300x225.jpg 300w, https:\/\/sc.qu.edu.iq\/wp-content\/uploads\/sites\/41\/2018\/04\/image-0-02-04-3e1015e47bca414abd932ee49fae576a84f8849c0d70c3721bc65f7918d3def3-V-600x450.jpg 600w, https:\/\/sc.qu.edu.iq\/wp-content\/uploads\/sites\/41\/2018\/04\/image-0-02-04-3e1015e47bca414abd932ee49fae576a84f8849c0d70c3721bc65f7918d3def3-V-768x576.jpg 768w, https:\/\/sc.qu.edu.iq\/wp-content\/uploads\/sites\/41\/2018\/04\/image-0-02-04-3e1015e47bca414abd932ee49fae576a84f8849c0d70c3721bc65f7918d3def3-V.jpg 1280w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/> <img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-7976\" src=\"https:\/\/sc.qu.edu.iq\/\/wp-content\/uploads\/2018\/04\/image-0-02-04-53e6b9dde28bb89a6eb669ff526f5604d4f706bd0cda15217108fb8bb9383a6b-V-300x225.jpg\" alt=\"\" width=\"300\" height=\"225\" srcset=\"https:\/\/sc.qu.edu.iq\/wp-content\/uploads\/sites\/41\/2018\/04\/image-0-02-04-53e6b9dde28bb89a6eb669ff526f5604d4f706bd0cda15217108fb8bb9383a6b-V-300x225.jpg 300w, https:\/\/sc.qu.edu.iq\/wp-content\/uploads\/sites\/41\/2018\/04\/image-0-02-04-53e6b9dde28bb89a6eb669ff526f5604d4f706bd0cda15217108fb8bb9383a6b-V-600x450.jpg 600w, https:\/\/sc.qu.edu.iq\/wp-content\/uploads\/sites\/41\/2018\/04\/image-0-02-04-53e6b9dde28bb89a6eb669ff526f5604d4f706bd0cda15217108fb8bb9383a6b-V-768x576.jpg 768w, https:\/\/sc.qu.edu.iq\/wp-content\/uploads\/sites\/41\/2018\/04\/image-0-02-04-53e6b9dde28bb89a6eb669ff526f5604d4f706bd0cda15217108fb8bb9383a6b-V.jpg 1280w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/> <img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-7977\" src=\"https:\/\/sc.qu.edu.iq\/\/wp-content\/uploads\/2018\/04\/image-0-02-04-ae40585f43dd26faf5e1af199434b3d1b5b423450e7106fc70cfd2a34e2a7978-V-300x225.jpg\" alt=\"\" width=\"300\" height=\"225\" srcset=\"https:\/\/sc.qu.edu.iq\/wp-content\/uploads\/sites\/41\/2018\/04\/image-0-02-04-ae40585f43dd26faf5e1af199434b3d1b5b423450e7106fc70cfd2a34e2a7978-V-300x225.jpg 300w, https:\/\/sc.qu.edu.iq\/wp-content\/uploads\/sites\/41\/2018\/04\/image-0-02-04-ae40585f43dd26faf5e1af199434b3d1b5b423450e7106fc70cfd2a34e2a7978-V-600x450.jpg 600w, https:\/\/sc.qu.edu.iq\/wp-content\/uploads\/sites\/41\/2018\/04\/image-0-02-04-ae40585f43dd26faf5e1af199434b3d1b5b423450e7106fc70cfd2a34e2a7978-V-768x576.jpg 768w, https:\/\/sc.qu.edu.iq\/wp-content\/uploads\/sites\/41\/2018\/04\/image-0-02-04-ae40585f43dd26faf5e1af199434b3d1b5b423450e7106fc70cfd2a34e2a7978-V.jpg 1280w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/> <img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-7978\" src=\"https:\/\/sc.qu.edu.iq\/\/wp-content\/uploads\/2018\/04\/image-0-02-04-d8bade9e9c2d8efc868864147e36189ab29338fc25c954badc1b4315f531a4ed-V-169x300.jpg\" alt=\"\" width=\"169\" height=\"300\" srcset=\"https:\/\/sc.qu.edu.iq\/wp-content\/uploads\/sites\/41\/2018\/04\/image-0-02-04-d8bade9e9c2d8efc868864147e36189ab29338fc25c954badc1b4315f531a4ed-V-169x300.jpg 169w, https:\/\/sc.qu.edu.iq\/wp-content\/uploads\/sites\/41\/2018\/04\/image-0-02-04-d8bade9e9c2d8efc868864147e36189ab29338fc25c954badc1b4315f531a4ed-V-338x600.jpg 338w, https:\/\/sc.qu.edu.iq\/wp-content\/uploads\/sites\/41\/2018\/04\/image-0-02-04-d8bade9e9c2d8efc868864147e36189ab29338fc25c954badc1b4315f531a4ed-V-600x1067.jpg 600w, https:\/\/sc.qu.edu.iq\/wp-content\/uploads\/sites\/41\/2018\/04\/image-0-02-04-d8bade9e9c2d8efc868864147e36189ab29338fc25c954badc1b4315f531a4ed-V.jpg 720w\" sizes=\"auto, (max-width: 169px) 100vw, 169px\" \/><\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u00a0\u0639\u0644\u0649 \u0642\u0627\u0639\u0629 \u0623\u0628\u0646 \u0627\u0644\u0647\u064a\u062b\u0645 \u0641\u064a \u0643\u0644\u064a\u0629 \u0627\u0644\u0639\u0644\u0648\u0645 \u0628\u062c\u0627\u0645\u0639\u0629 \u0627\u0644\u0642\u0627\u062f\u0633\u064a\u0629 \u062c\u0631\u062a \u0645\u0646\u0627\u0642\u0634\u0629 \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u0627\u0644\u0645\u064f\u0639\u064e\u062f\u0629 \u0645\u0646 \u0642\u0628\u0644 \u0627\u0644\u0637\u0627\u0644\u0628\u0629 \u0639\u0637\u0627\u0621 \u062e\u0644\u064a\u0644 \u062d\u0633\u064a\u0646 \u062a\u062d\u062a &#8230; <a class=\"cz_readmore cz_readmore_no_icon\" href=\"https:\/\/sc.qu.edu.iq\/?p=7974\"><span>\u0627\u0642\u0631\u0623 \u0623\u0643\u062b\u0631<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":7977,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"chat","meta":{"footnotes":""},"categories":[5,654],"tags":[],"class_list":["post-7974","post","type-post","status-publish","format-chat","has-post-thumbnail","hentry","category-5","category-654","post_format-post-format-chat"],"_links":{"self":[{"href":"https:\/\/sc.qu.edu.iq\/index.php?rest_route=\/wp\/v2\/posts\/7974","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sc.qu.edu.iq\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sc.qu.edu.iq\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sc.qu.edu.iq\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/sc.qu.edu.iq\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=7974"}],"version-history":[{"count":1,"href":"https:\/\/sc.qu.edu.iq\/index.php?rest_route=\/wp\/v2\/posts\/7974\/revisions"}],"predecessor-version":[{"id":22123,"href":"https:\/\/sc.qu.edu.iq\/index.php?rest_route=\/wp\/v2\/posts\/7974\/revisions\/22123"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sc.qu.edu.iq\/index.php?rest_route=\/wp\/v2\/media\/7977"}],"wp:attachment":[{"href":"https:\/\/sc.qu.edu.iq\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=7974"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sc.qu.edu.iq\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=7974"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sc.qu.edu.iq\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=7974"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}